GE PVDF (Polyvinylidene Fluoride) Transfer Membranes

Product Information

Naturally hydrophobic unsupported membrane Chemical compatibility allows the use of all commonly used stains Low backgrounds, high sensitivity Highest binding capacity eliminating sample "blow through" BSA binding capacity is 125 µg/cm²

Applications

• Western transfers
• Protein sequencing
• Peptide mapping
• Amino acid analysis
• Dot/slot blots

Products

Part # Model # Description Pore Size Filter Size Qty. Price 1214429 PV2HY00010 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.22 micron 300x3000 mm 1 $271 1215032 PV2HY320F5 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.22 micron 200x200 mm 5 $114 1214726 PV2HYA0010 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.22 micron 200x3000 mm 1 $183 1214842 PV2HYB0010 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.22 micron 150x3000 mm 1 $140 1212639 PV4HY00010 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 300x3000 mm 1 $271 1212644 PV4HY310F0 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 100x100 mm 10 $106 1212636 PV4HY315F5 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 150x150 mm 5 $75 1214802 PV4HY320F2 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 200x200 mm 25 $422 1212637 PV4HY320F5 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 200x200 mm 5 $114 1212643 PV4HY415F0 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 100x150 mm 10 $114 1214558 PV4HY910F0 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 90x100 mm 10 $103 1212642 PV4HY912F0 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 90x120 mm 10 $106 1212783 PV4HYA0010 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 200x3000 mm 1 $183 1212781 PV4HYB0010 GE PVDF (Polyvinylidene Fluoride) Transfer Membrane 0.45 micron 150x3000 mm 1 $140

Product Description

GE PVDF is a naturally hydrophobic, unsupported polyvinylidene fluoride transfer membrane designed, manufactured and tested by GE Osmonics to deliver the highest binding capacity combined with low backgrounds in western transfer, amino acid analysis and protein sequencing applications. Exceptional quality control procedures throughout the manufacturing process eliminates "lot-to-lot" variation and greatly minimizes "bald spot" problems. PVDF assures reproducible results with maximum sensitivity.

Protein can be electroblotted from a variety of gel matrices onto GE PVDF. Because of its high sensitivity and strong binding affinity, protein will not pass through the membrane. The broad chemical compatibility of GE PVDF allows the use of all commonly used stains such as: Amido Black, Colloidal Gold, Coomassie Blue, India Ink and Ponceau-S. GE PVDF will not degrade, distort or shrink when using high concentrations of methanol for destaining. The exceptional tensile strength allows for multiple reprobings and easy removal of target bands without concern for the membrane tearing, fracturing or curling.

To assure you of the highest binding and to eliminate sample "blow through", regardless of how small your peptides may be, GE Osmonics offers three pore size ratings. As standard product, GE Osmonics offers 0.22 and 0.45 micron pore sizes in all sheet and roll configurations.

GE PVDF exhibits efficient transfer of proteins in a wide range of molecular weights, from 5k Daltons to 700k Daltons. The binding capacity averages 125 µg/cm² for larger globular proteins such as immunoglobulins and higher binding capacities for small peptides.

For Additional Information

Please call GE Osmonics' Labstore Support at (800) 444-8212, by fax (952) 988-6662 or email us for questions, pricing and availability. For international support and distributor locations, call +1-952-988-6665 or email us. Many of the standard technical questions are already answered in our Frequently Asked Questions.