GE Nitrocellulose Supported Transfer Membranes

Product Information

Supported for procedures requiring rigorous handling Strong - will not curl, bend or crack after baking High sensitivities, low backgrounds Multiple reprobings Nucleic acid binding is 100 µg/cm²

Applications

• Northerns, Southerns
• Multiple re-hybridizations
• Colony/plaque lifts
• Dot/slot blots
• Radiolabelled detection systems
• Chemiluminescent
• Biotinylated detection systems

Products

Part # Model # Description Pore Size Filter Size Qty. Price 1212632 WP2HY00010 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 300x3000 mm 1 $277 1212640 WP2HY08250 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 82 mm 50 $123 1212837 WP2HY08550 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 85 mm 50 $124 1214560 WP2HY310F0 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 100x100 mm 10 $80 1212669 WP2HY315F5 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 150x150 mm 5 $62 1212689 WP2HY320F5 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 200x200 mm 5 $109 1213195 WP2HY330F5 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 300x300 mm 5 $159 1213141 WP2HY450F5 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 300x500 mm 5 $229 1222287 WP2HY912F0 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 90x120 mm 10 $80 1212690 WP2HYA0010 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 200x3000 mm 1 $187 1212721 WP2HYB0010 GE Nitrocellulose Supported Transfer Membrane 0.22 micron 150x3000 mm 1 $142 1212590 WP4HY00010 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 300x3000 mm 1 $251 1212591 WP4HY08250 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 82 mm 50 $113 1212592 WP4HY08550 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 85 mm 50 $114 1212594 WP4HY13250 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 132 mm 50 $218 1214248 WP4HY13700 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 137 mm 100 $333 1212595 WP4HY13750 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 137 mm 50 $223 1212596 WP4HY315F5 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 150x150 mm 5 $59 1214762 WP4HY320F2 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 200x200 mm 25 $368 1212597 WP4HY320F5 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 200x200 mm 5 $100 1212598 WP4HY322F5 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 220x220 mm 5 $104 1212599 WP4HY330F5 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 300x300 mm 5 $145 1212600 WP4HY3MTF0 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 102x133 mm 10 $95 1214978 WP4HY408F0 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 70x84 mm 10 $57 1215319 WP4HY410F0 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 70x100 mm 10 $63 1214935 WP4HY417F2 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 140x160 mm 25 $293 1212601 WP4HY450F5 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 300x500 mm 5 $210 1212602 WP4HYA0010 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 200x3000 mm 1 $171 1212604 WP4HYB0010 GE Nitrocellulose Supported Transfer Membrane 0.45 micron 150x3000 mm 1 $131

Product Description

GE Nitrocellulose - is an internally supported nitrocellulose membrane that combines the binding characteristics of nitrocellulose with the strength of nylon. GE Nitrocellulose - supported is ideal for applications where nitrocellulose has been used in the past such as DNA / RNA /Protein transfers. However, it outperforms unsupported nitrocellulose when extensive handling is required such as reprobing or colony and plaque lifts.

GE Osmonics' unique process of impregnating pure nitrocellulose polymer onto an inert polyester web produces a very strong, dimensionally stable hybridization membrane. The inert web provides dimensional strength to prevent curling, cracking and tearing even after baking. This ensures the highest binding nitrocellulose membrane, approximately 100 µg/cm². There is nothing to lower the binding capacity such as cellulose acetate or other additives.

This combination is especially important in colony and plaque lifts and multiple reprobings or any other procedure requiring rigorous handling. Membrane breakage is eliminated, reducing the cost due to wasted time, materials, and lost data. Many researchers have reprobed GE Nitrocellulose - supported as many as five times.

In all protocols using nitrocellulose, GE Nitrocellulose - supported may be substituted without making any changes in the protocol while maintaining the same high signal and low backgrounds.

All membranes are Triton free and do not contain any other cytotoxins.

For Additional Information

Please call GE Osmonics' Labstore Support at (800) 444-8212, by fax (952) 988-6662 or email us for questions, pricing and availability. For international support and distributor locations, call +1-952-988-6665 or email us. Many of the standard technical questions are already answered in our Frequently Asked Questions.