Recipes for Buffers used in Nucleic Acid Transfers

Electrophoresis Buffers:

1. TAE - (1x)    40 mM Tris-acetate    1mM EDTA, pH     8.0 pH adjustments can be made with glacial acetic acid
   

2. TBE - (1x)    45mM Tris-base    45mM boric acid     2mM EDTA, pH 8.0
   

3. MOPS Buffer - (10x)    50mM sodim acetate    10mM EDTA     0.2M 3-morpholinopropanesulfonic acid, pH 7.0
   

4. E Buffer    5mM sodium borate    10mM sodium sulfate     1mM EDTA    50mM boric acid, pH 8.2

Transfer and Washing Buffers:

1. SSPE (1x)    0.18M NaCl    10mM sodium phosphate, pH 7.7    1mM EDTA    To bring pH to 7.7, Na₂HPO₄ is added to NaH₂PO₄
   

2. SSC (1x)    0.15M NaCl    15mM sodium citrate, pH 7.0
   

3. TBS (1x)    50mM Tris    150mM NaCl

Blocking Agents:

1. Denhardt's Reagent    0.02% Ficoll (mw = 400,000)     0.02% polyvinylpyrolidone (mw = 40,000) 0.02% BSA                                                                                                          (Bovine Serum Albumin)

Sample Buffers

1. TE Buffer (1x)    10mM Tris-HCl, pH 8.0    1mM EDTA
      

2. SET Buffer (1x)    0.1M NaCl    10mM Tris-HCl, pH 8.0     1mM EDTA

*pH adjustment to EDTA are made with NaOH
*pH adjustments to Tris solution is made with HCl

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